The Line Probe assay is used for the diagnosis of MDR-TB. It can be done on sputum directly or on culture. What sample was used to diagnose the TB and what was the reading on the bands. Principle of Line Probe Assay Line probe assays are a family of DNA strip-based tests that determine the drug resistance profile of a MTBC strain through the pattern of binding of amplicons DNA amplification products to probes targeting the most common resistance associated.
Feb 10, 2016 · The MTBDRplus and MTBDRsl line probe assays were evaluated in 181 sputum samples and 270 isolates from patients with culture-confirmed drug-sensitive-TB, MDR-TB, or XDR-TB. Phenotypic culture. Line probe assay LPA was performed directly on the sputum specimens and the results were compared with that of conventional drug susceptibility testing DST on. Jan 01, 2017 · Line probe assays LPAs enable rapid drug-susceptibility testing for rifampicin RIF and isoniazid INH resistance and Mycobacterium tuberculosis detection. Genotype MTBDRplusV1 was WHO-endorsed in 2008 but newer LPAs have since been developed. clinical specimens, such as sputum [4,5]. Among the molecu-lar assays, line probe assays have shown great promise . Line probe assays are a family of novel DNA strip-based tests that use nucleic acid amplification techniques e.g., PCR and reverse hybridization methods for the rapid detection of mutations asso - ciated with drug resistance. line probe assays and the time, space and resources required for such renovations should be carefully considered. 3.4 Electrical supply and back-up power Reagents used in line probe assays must be refrigerated or frozen, while amplification and hybridization procedures must be conducted under closely monitored temperature conditions.
Jul 28, 2005 · Line probe assay has been shown to be highly sensitive and specific in the detection of rifampicin-resistant TB when used on culture isolates. There is a paucity of data on application of this test directly to clinical specimens, although based on a small number of. Apr 01, 2014 · Limited performance data from line probe assays LPAs, nucleic acid tests used for the rapid diagnosis of tuberculosis TB, nontuberculosis mycobacteria NTM, and Mycobacterium tuberculosis drug resistance are available for HIV-infected individuals, in whom paucibacillary TB is common. In this study, the strategy of testing sputum with GenoType MTBDRplus MTBDR-Plus and. Line probe assays LPAs have been recently approved for use in low income settings and can be used to screen smear-positive sputum specimens for resistance to rifampicin and.
The utility of a line probe assay Genotype MTBDRplus performed directly on 2-month sputa to monitor tuberculosis treatment response is unknown. We assessed if direct testing of 2-month sputa with MTBDRplus can predict 2-month culture conversion and long-term treatment outcome. Oct 15, 2019 · The line probe assay LPA, based on strip technology was used to diagnose TB and detect RIF as well as Isoniazid INH resistance due to mutations in rpoβ, and both inhA and katG genes. The test was performed according to the manufacturer’s protocol Hain Life.
needs. The recommended use of line probe assays is currently limited to M. tuberculosis isolates and direct testing of smear-positive sputum specimens. Line probe assays are not a complete replacement for conventional culture and DST, as mycobacteriological culture is still. Your Competent Partner in Mycobacteria Diagnostics and Instrumentation No matter if you want to start with molecular genetic testing, expand your existing mycobacteria diagnostics or look for an easy way to automate the processing: We have the appropriate solutions for all your needs! Jan 04, 2016 · The line-probe assay uses the reverse hybridization technology.c DNA-probes immobilized in parallel lines on a paper strip. The target DNA, previously extracted by boiling, is PCR-amplified using biotinylated primers and finally incubated with the strip.
MOLECULAR LINE-PROBE ASSAY FOR THE DETECTION OF RESISTANCE TO ISONIAZID AND RIFAMPICIN LPA TUBERCULOSIS DIAGNOSTICS BACKGROUND Multidrug-resistant tuberculosis MDR TB is a public health crisis and a major threat to global TB control. Globally in 2015, there were 4 80 000 new cases of. treatment. There is thus need for a rapid, reliable and easily accessible assay. This comparative analysis was performed to assess diagnostic performance characteristics of GeneXpert MTB/RIF and Line Probe Assay LPA. Methods: Three hundred twenty nine sputum samples of patients across the 47 counties in Kenya suspected to. The Genotype MTBDRplus test is a WHO-endorsed line-probe assay LPA for the rapid detection of Mycobacterium tuberculosis complex MTB and mutations conferring resistance to rifampin RIF and isoniazid INH in AFB smear-positive sputum specimens. Sep 01, 2012 · The sample size of 50 AFB staining positive sputum samples taken was tested. Of these 30% were cases known to be MDR cases as detected from culture tests and 70% were fresh smear positive cases on treatment for TB. The Line probe assays were developed to cover common drug resistant mutations with rifampicin, INH, and aminoglycosides.
Feb 26, 2010 · In 2008 in Kampala, MDR-TB prevalence was reported as 1.0% and 12.3% in new and previously treated TB cases respectively. Line probe assays LPAs have been recently approved for use in low income settings and can be used to screen smear-positive sputum specimens for resistance to rifampicin and isoniazid in 1-2 days. GLI guide for the interpretation and reporting of line probe assays: This guide has been developed to provide practical information on the interpretation of the most commonly used first- and second-line line probe assays LPA. In addition to the interpretation of the mutations identified by the two assays, this guide includes practical recommendations on additional follow up diagnostic. What is Line Probe Assay LPA? • It is a test that diagnoses TB and simultaneously detects resistance to RIF and INH. • It does this by detecting the presence of the DNA of Mycobacterium tuberculosis in the sputum and also identifies any changes/ mutations in the. Objectives. The development of rapid molecular diagnostic assays for pyrazinamide PZA resistance is considered technically challenging as mutations are highly diverse, scattered along the full length of the pncA gene and not all are associated with PZA resistance. We evaluated the performance of the novel Genoscholar PZA-TB II line probe assay PZA-LPA2; NIPRO Corporation, Japan. Diagnostic performance of genoType ® MTBDRplus line probe assay in bronchoalveolar lavage for pulmonary tuberculosis diagnosis in sputum scarce and smear-negative patients Farah Idrees 1, Muhammad Irfan 1, Kauser Jabeen 2, Joveria Farooqi 2, Rumina Hasan 2 1 Department of Medicine, Aga Khan University, Karachi, Pakistan 2 Department of Pathology and Laboratory Medicine, Aga Khan.
Evaluation of genotype MTBDRplus VER 2.0 line probe assay for the detection of MDR-TB in smear positive and negative sputum samples. The study purpose was to validate the use of the GenoType MTBDRplus line probe assay LPA to detect resistance to R and H in Mycobacterium tuberculosis strains directly from smear-positive sputum. as liquid culture, line probe assay, Xpert MTB/RIF overcome many of the limitations of smear microscopy, especially for patients living with HIV/AIDS and those with a high likelihood of having drug-resistant TB. WHO and The Union have previously published guidelines for sputum smear microscopy.
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